High contrast imaging of reversibly switchable fluorescent proteins via temporally unmixed Multispectral Optoacoustic Tomography (tuMSOT)

摘要

Photocontrol of reversibly switchable fluorescent proteins (RSFPs) was usedto program optoacoustic signal time courses that were temporally unmixed toincrease the proteins contrast-to-noise-ratios (CNRs) in optoacoustic imaging.In this way, two variants of the RSFP Dronpa with very similar optoacousticspectra could be readily discriminated in the presence of highly absorbingblood. Addition of temporal unmixing to multispectral optoacoustic tomography(tuMSOT) in conjunction with synthetic or genetically controlled photochromiccontrast agents and customized photoswitching schedules can increase theperformance of multiplexed and high contrast molecular optoacoustic imaging.